SidaGhanaV1, Main, Exploration, bibRecord, 001567

Isolation and characterization of HIV-2 from an AIDS patient in Ghana.

Identifieur interne : 001567 ( Main/Exploration ); précédent : 001566; suivant : 001568

Isolation and characterization of HIV-2 from an AIDS patient in Ghana.

Auteurs : K. Ishikawa [Japon] ; H. Tsujimoto ; M. Nakai ; J A Mingle ; M. Osei-Kwasi ; S E Aggrey ; V B Nettey ; S N Afoakwa ; M. Fukasawa ; T. Kodama

Source :

AIDS (London, England) [ 0269-9370 ] ; 1988.

RBID : pubmed:3146268

Descripteurs français

KwdFr :
- ARN viral (isolement et purification), Ghana, Humains, Hybridation d'acides nucléiques, Protéines des retroviridae (isolement et purification), Syndrome d'immunodéficience acquise (microbiologie), Technique de Western, VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (isolement et purification).
MESH :
- isolement et purification : ARN viral, Protéines des retroviridae, VIH-2 (Virus de l'Immunodéficience Humaine de type 2).
- microbiologie : Syndrome d'immunodéficience acquise.
- Ghana, Humains, Hybridation d'acides nucléiques, Technique de Western.
Wicri :
- geographic : Ghana.

English descriptors

KwdEn :
- Acquired Immunodeficiency Syndrome (microbiology), Blotting, Western, Ghana, HIV-2 (isolation & purification), Humans, Nucleic Acid Hybridization, RNA, Viral (isolation & purification), Retroviridae Proteins (isolation & purification).
MESH :
- chemical , isolation & purification : RNA, Viral, Retroviridae Proteins.
- geographic : Ghana.
- isolation & purification : HIV-2.
- microbiology : Acquired Immunodeficiency Syndrome.
- Blotting, Western, Humans, Nucleic Acid Hybridization.

Abstract

This report describes the isolation and characterization of a retrovirus of the HIV-2 group from a Ghanaian AIDS patient which has different restriction patterns from previously reported HIV-2 viruses. The virus was morphologically very similar to HIV-1 and HIV-2, and had Mg2+-dependent reverse transcriptase. Like previous HIV isolates, it induced severe cytopathic effects in CD4-positive human lymphoid cell lines. Its major proteins were shown to be gp110, p66, p55, p41, gp32, p30 and p26 by Western blot analysis. In dot-blot hybridization experiments, the virus hybridized with a HIV-2 DNA probe, but not with HIV-1 and SIVagm probes in stringent conditions. These data indicate that this Ghanaian virus is a HIV-2 group virus. However, in a Southern blot hybridization experiment, the restriction patterns of this virus, designated HIV-2 [GH-1], were quite different from those of previously reported HIV-2 viruses from West Africa isolated at the Pasteur Institute.

PubMed: 3146268

Affiliations:

Japon

Le document en format XML

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<front><div type="abstract" xml:lang="en">This report describes the isolation and characterization of a retrovirus of the HIV-2 group from a Ghanaian AIDS patient which has different restriction patterns from previously reported HIV-2 viruses. The virus was morphologically very similar to HIV-1 and HIV-2, and had Mg2+-dependent reverse transcriptase. Like previous HIV isolates, it induced severe cytopathic effects in CD4-positive human lymphoid cell lines. Its major proteins were shown to be gp110, p66, p55, p41, gp32, p30 and p26 by Western blot analysis. In dot-blot hybridization experiments, the virus hybridized with a HIV-2 DNA probe, but not with HIV-1 and SIVagm probes in stringent conditions. These data indicate that this Ghanaian virus is a HIV-2 group virus. However, in a Southern blot hybridization experiment, the restriction patterns of this virus, designated HIV-2 [GH-1], were quite different from those of previously reported HIV-2 viruses from West Africa isolated at the Pasteur Institute.</div>
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Le SIDA au Ghana (serveur d'exploration)

Isolation and characterization of HIV-2 from an AIDS patient in Ghana.

Isolation and characterization of HIV-2 from an AIDS patient in Ghana.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Le SIDA au Ghana (serveur d'exploration)
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